Free HPLC & LC-MS calculators
21 calculators for method development, system suitability, quantitation, validation and LC-MS. Each opens with a worked example and explains the formula behind it.
Free learning apps & Chromie Academy
Full interactive apps and learning tools, free to use. They open in a new tab.
Lifecycle Assist Pro: free demoFrom structure to method, forced degradation, validation and shelf life. Four worked molecules, no key needed.Open free app ↗Molecule ExplorerLook up any PubChem compound: ionised fraction, logD and speciation across pH, plus a starting method.Open free app ↗HPLC Method OptimizerExplore how method conditions change your separation.Open free app ↗Chromie Academy: HPLC method developmentFree interactive HPLC method development learning, step by step.Open free app ↗Chromie Academy: Pharma regulatory essentialsICH, GMP, stability, impurities, data integrity and post-approval changes in eight short modules, with a certificate.Open free app ↗HPLC Method Validation learning toolWork through ICH Q2(R2) validation parameters.Open free app ↗LC-MS Method Development learning toolIonisation, MRM tuning and LC-MS method building.Open free app ↗LC-MS Method Validation learning toolBioanalytical validation to ICH M10, explained.Open free app ↗
System suitability & separation
Check that a run is fit for purpose.
Resolution, plates & tailingCheck a critical pair against USP <621> system suitability.Open calculatorSignal-to-noise (S/N)Measure S/N the pharmacopoeial way and estimate LOD and LOQ from one injection.Open calculatorVoid time, k & selectivityEstimate t0 from column size and flow, then check k and α for two peaks.Open calculator
Method development
Choose pH, buffer, gradient and column conditions.
pH vs pKa ionisationSee how much of the analyte is ionised, and whether small pH errors will move its retention.Open calculatorBuffer preparationGet the weights (or volumes) of each buffer component for a target pH.Open calculatorGradient designerCheck whether a gradient is too steep, and how long to hold and re-equilibrate.Open calculatorDwell volume compensationKeep retention times when a gradient method moves to an instrument with a different dwell volume.Open calculatorColumn scalingScale flow, injection volume and gradient time when column dimensions change.Open calculatorBackpressure & viscosityEstimate column pressure before you switch to smaller particles or a higher flow.Open calculator
Quantitation
Turn peak areas into results.
Assay % (external standard)Turn sample and standard peak areas into % content or % label claim.Open calculatorImpurity % with RRF & ICH thresholdsQuantify an impurity against the API standard and see which ICH Q3B threshold it crosses.Open calculatorNitrosamine limit (AI to ppm)Convert an acceptable intake into a ppm limit for your dose and check several nitrosamines, including CPCA categories.Open calculatorStandard & dilution prepGet the true stock concentration and the aliquot for your working standard.Open calculator
Validation statistics
Linearity, precision, limits and mass balance.
Linearity & regressionPaste your calibration levels and get the regression, r² and back-calculated accuracy.Open calculatorPrecision (%RSD)Paste replicate injections or results and check %RSD against your limit.Open calculatorLOD & LOQ from calibrationCalculate detection and quantitation limits from the calibration slope and response SD.Open calculatorForced degradation mass balanceCheck that assay loss is accounted for by the degradants you can see.Open calculator
LC-MS
Masses, adducts and matrix effects.
Exact mass & adduct m/zType a molecular formula and get the exact mass and the m/z of common ESI adducts.Open calculatorMass accuracy (ppm)Check measured against theoretical m/z, and set an extraction window.Open calculatorMatrix effect, recovery & process efficiencySeparate ion suppression from extraction loss with the three-set (A, B, C) design.Open calculator
Lab planning
Prepare solutions and mobile phases.
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