Free HPLC and LC-MS calculators, professional apps, guides and templates for scientists who develop, validate and troubleshoot methods. Formulas referenced to USP <621>, Ph. Eur. and ICH.
Mirela Gorjanu, Founder · 20+ years in the pharmaceutical industry
Short, narrated lessons built from animation, not a talking head. Watch the band spread in the column, the ions form in the source and the peaks move as you turn each knob. Each course ends with a capstone and a final exam, and comes with a companion book.
10 modules · 64 lessons · 3 h 9 min
From how a separation works to troubleshooting, QbD method development, robustness, validation to ICH Q2(R2) and data integrity.
Buy it once and it is yours to keep · companion book included
Free lesson: 1.3 Inside the column
10 modules · 50 lessons · 2 h 3 min
Ionisation, mass analysers, MRM tuning, sample preparation, matrix effects and full ICH M10 bioanalytical validation.
Buy it once and it is yours to keep · companion book included
Free lesson: 6.2 Post-column infusion
9 modules · 44 lessons · 1 h 35 min
Columns, inlets, temperature programming, EI spectra and library searching, SIM quantitation, validation and troubleshooting.
Buy it once and it is yours to keep · companion book included
Free lesson: 8.3 Troubleshooting the inlet
Want all three? Get the HPLC, LC-MS and GC-MS courses with all three books in one set. Get the complete set
Organised by the job you are doing. Each tool opens with a worked example and explains the theory behind it.
Check that a run is fit for purpose.
Choose pH, buffer, gradient and column conditions.
Turn peak areas into results.
Linearity, precision, limits and mass balance.
Masses, adducts and matrix effects.
Full interactive apps and learning tools, free to use with no key needed.
The questions analytical chemistry interviews actually ask, with clear answers.
Browser-based apps that take you further than a single calculation. Nothing to install. Pick a stage to see the apps for it.
Planning and teaching tools. They are not validated computerised systems and do not produce controlled GxP records.
Illustrated guides, slide courses, validation templates and interactive simulators. Start with a bundle, or pick one topic.
HPLC Method Development, Step by StepA practical route for every analyte type.
HPLC Assay Method ValidationThe complete ICH-aligned reference.
HPLC Peak Separation DecodedResolution, selectivity and co-elution solved.
The Complete HPLC Visual Reference155+ illustrated guides across the workflow.
Illustrated HPLC Comprehensive GuideThe whole technique, explained visually.
The Theory of Separation in HPLCWhy peaks separate, in plain language.
The HPLC Survival GuideThe lab detective’s handbook for real problems.
RP-HPLC Column Selection SimulatorCompare 10 stationary phases on the same compounds.
HPLC Gradient Method Development SimulatorSee how gradient shape moves your peaks.
HPLC Method Development SimulatorChange pH, gradient and column length and watch the result.
Molecular Chemistry on C18 SimulatorHow different chemistries behave on a C18 column.Short lessons on HPLC, LC-MS and method validation, plus walkthroughs of the apps.
“Most HPLC problems start before the first injection: a pH picked without the analyte’s pKa, a gradient copied from a different column, a validation plan written after the method is already fixed.”
I’m an analytical and bioanalytical chemist with more than 20 years in pharmaceutical R&D across Canada and Europe, in method development, validation, regulatory compliance and quality control in GMP and GLP environments.
I founded Practical Pharma Science to give analysts the tools I wanted at the bench: calculators that show their working, apps that follow the guidelines auditors use, and guides written in plain language.
Smarter HPLC Method Development: Applied Chromatographic Intelligence
New lessons, tools and guides go out on these channels first.
The most-used check in chromatography: Rs, plates and tailing against USP <621>.
